affinity chromatography

基本解释

Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography.
多克隆抗体通过用DYKDDDDK合成肽免疫动物制备。抗体用蛋白A和多肽亲和色谱法纯化。
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In the present study, recombinant human IRF-7 was expressed in Escherichia coli, purified by affinity chromatography, and utilized to raise polyclonal antibody in BALB/c mice.
本文在大肠杆菌中表达了重组IRF-7，利用亲和层析的方法进行了纯化，并制备了鼠抗IRF-7的多克隆抗体。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。

亲和层析
We isolated the LN receptor from the tissue of Lewis lung carcinoma by affinity chromatography.
本文采用亲和层析法从小鼠Lewis肺癌组织中分离LN受体并对其理化性质进行研究。
亲和色谱
亲和纯化
The His-ABPG fusion protein was obtained using the affinity chromatography and in a size of about 68.5 kDa.
利用表达质粒上的His融合标签进行亲和纯化,获得电泳纯融合蛋白,其大小约为68.5 kDa。
亲和色谱法

亲和层析
The NS1 protein was acquired by the purification of chitin affinity chromatography.
经几丁质柱亲和层析纯化得到NS1蛋白。
亲和色谱
Methods We added perchloric acid into human plasma. Apolipoprotein H was separated and purified by ion exchange chromatography and affinity chromatography.
方法用高氯酸预处理血浆,以离子交换色谱和亲和色谱相结合的方法进行蛋白质的分离和纯化。
受体色谱